Minimum Essential Medium Eagle

簡(jiǎn)要描述：Minimum Essential Medium Eagle
With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

產(chǎn)品型號：型號齊全
廠(chǎng)商性質(zhì)：代理商
更新時(shí)間：2023-07-11
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詳情介紹

Minimum Essential Medium Eagle：

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:HEPES: no;NaHCO3: yes;L-glutamine: yes;Earle’s salts (5% CO2): yes;sodium pyruvate: no;

phenol red: yes.General description:Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to c*te normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for c*tion of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application:Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

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Minimum Essential Medium Eagle

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